Molecular Detection of Some Vancomycin and Virulence Factor Genes in Enterococcus faecium

Multidrug-resistant (MDR) and pathogenic E. faecium is a crisis in healthcare settings. This survey aimed at antibiotic susceptibility profiling virulence determinants of faecium. study pooled 100 fecal isolates identified by phenotypic molecular tests. Antibiotic ampicillin MIC were determined according to clinical laboratory standards institute (CLSI) 2017. Moreover, biofilm formation was assayed microtiter tissue plate assay. Virulence genes pattern performed polymerase chain reaction (PCR) method. Among 460 samples, identified, among which the highest resistance related penicillin (81%), cephalothin (78%), cefazolin (76%), tetracycline cefepime (69%). In contrast, 83% them susceptible vancomycin. four vancomycin-resistant had vancomycin MIC>32µg/mL, 11 MIC>8µg/mL. Of 32 ampicillin-resistant isolates, 18 (56%) produced strong biofilm, 14 (44%) moderate biofilm. 17 (VREfa), 15 (88.23%) biofilms, two moderate-level significantly higher than (p=0.0144). The vanA (vancomycin MIC: 16-64µg/mL) vanB 8-64µg/mL) detected twelve five respectively. rate adhesin ace, esp ebp included 68%, 97% 82%, All VREfa harbored genes. low. Biofilm different between vancomycin-susceptible but not k9ampicillin-resistant ampicillin-susceptible isolates. associated with formation. Further studies are essential appreciate better relation formation, drug non-susceptibility Keywords: faecium, resistance,